Journal: JCI Insight

Article Title: T cell developmental arrest in former premature infants increases risk of respiratory morbidity later in infancy

doi: 10.1172/jci.insight.96724

Figure Lengend Snippet: A subset of 16 study samples was stained to explore the relationship between CD31 and cytokine function in umbilical cord blood. t-SNE was performed within the Flowjo package. (A) Event distribution based on nearest-neighbor analysis and walk through of the IL-8+/TNF-α neighborhood are shown within the circle. (B) Progression of CD31, IL-2, IL-8, and TNF-α (C) and 2-dimensional plots demonstrating a phenotypic continuum associated with age cohort while moving through local structure. t-SNE, t-stochastic neighbor embedding. This figure is available as a Supplemental Video.

Article Snippet: To validate findings based on manual gating, an unsupervised, nonlinear dimensionality reduction of flow cytometry data (staining panel 3, Supplemental Table 2 ) was performed using the t-distributed stochastic neighbor embedding (t-SNE) dimensionality reduction algorithm (FlowJo v.10.2) ( 24 ). t-SNE measures the similarity between pairwise points (single cells) in high dimensional space and embeds them into a reduced, visually rich two-dimensional scatter plot in such a way that the local, high dimensional structure of the single-cell data is maintained.

Techniques: Staining